Journal: IET Nanobiotechnology

Article Title: Facile hydrothermal bio‐synthesis of cellulose acetate templated CuS nanorods like fibres: antibacterial, cytotoxicity effects and DNA cleavage properties against A549 lung cancer cells

doi: 10.1049/iet-nbt.2019.0193

Figure Lengend Snippet: Comparative analysis of nanostructured CuS NPs against inhibition of different cancer cells

Article Snippet: Ltd., Chennai for cell line lung cancer (A549) samples and for other biological activities.

Techniques: Inhibition

Journal: IET Nanobiotechnology

Article Title: Facile hydrothermal bio‐synthesis of cellulose acetate templated CuS nanorods like fibres: antibacterial, cytotoxicity effects and DNA cleavage properties against A549 lung cancer cells

doi: 10.1049/iet-nbt.2019.0193

Figure Lengend Snippet: % of inhibition of A549 cancer cells by anti‐proliferative assay

Article Snippet: Ltd., Chennai for cell line lung cancer (A549) samples and for other biological activities.

Techniques: Inhibition

Journal: IET Nanobiotechnology

Article Title: Facile hydrothermal bio‐synthesis of cellulose acetate templated CuS nanorods like fibres: antibacterial, cytotoxicity effects and DNA cleavage properties against A549 lung cancer cells

doi: 10.1049/iet-nbt.2019.0193

Figure Lengend Snippet: Microscopic images of A549 cancer cells upon treatment with IC50 concentration of biosynthesised CuS nanofibres at different concentration levels

Article Snippet: Ltd., Chennai for cell line lung cancer (A549) samples and for other biological activities.

Techniques: Concentration Assay

Journal: Biomolecules

Article Title: Planarian Mucus: A Novel Source of Pleiotropic Cytotoxic and Cytostatic Agents against Cancer Cells

doi: 10.3390/biom14091075

Figure Lengend Snippet: Effect of D. japonica Mucus Treatment on Cell Growth. ( a ) A representative experiment demonstrating the dose-dependent impact of mucus treatment on NCI-H358 and 16HBE14o cell numbers, assessed via crystal violet assay 48 h post-treatment. Each point reflects the mean value (±SD) of three independent samples, normalized to their respective controls. ** = p < 0.01, calculated using Student’s t -test. The inset displays EC50 values determined using the Graphpad Prism program. ( b ) This representative experiment illustrates the influence of various mucus preparations on NCI-H358 cell growth, evaluated through crystal violet assay 48 h post-treatment. Each bar represents the mean absorbance (±SD) of three independent samples. NS indicates not significant, determined by one-way ANOVA. ( c ) A representative experiment illustrating the dose-dependent impact of mucus treatment on cell numbers in A549, Caco-2, and THP-1 cells. Cell numbers were assessed using the crystal violet assay for A549 and Caco-2 cells, and direct counting for THP-1 cells, 48 h post-treatment. Each bar represents the mean value (±SD) of three independent samples, normalized to their respective controls. ** = p < 0.01, calculated by Student’s t -test. ( d ) Representative experiment, in which we observe the dose-dependent effect of mucus treatment on cell death, evaluated using the PI incorporation assay in A549, Caco-2, and THP-1 cells, 48 h post-treatment. Each bar represents the percentage of dead and alive cells (±SD) assessed in three independent samples. * = p < 0.05; ** = p < 0.01, determined by Student’s t -test.

Article Snippet: The human non-small-cell lung cancer cell line A549 and the human bronchioalveolar carcinoma cell line NCI-H358 were kindly provided by Doctor Laura Poliseno from the Italian National Research Council of Pisa.

Techniques: Crystal Violet Assay

Journal: Frontiers in Immunology

Article Title: A novel oncolytic Vaccinia virus armed with IL-12 augments antitumor immune responses leading to durable regression in murine models of lung cancer

doi: 10.3389/fimmu.2024.1492464

Figure Lengend Snippet: A novel tumor-selective oncolytic VVL-m12 replicates efficiently in and is cytotoxic to lung cancer cell lines. (A) Vaccinia virus Lister strain with deletions of the thymidine kinase (TK) and N1L genes has been described previously . We retained the original B5R gene and placed the signal peptide, stalk (S), transmembrane (T) and cytoplasmic tail (C) regions (STC) in the deleted four short consensus repeat (SCR) domains under an H5 promoter control within the TK region to obtain VV CTRL . IL-12 (murine (m) or human (h)) was incorporated into the N1L region of VV CTRL under control of the H5 promoter, creating VVL-m/h12. (B) Cytotoxicity of VVLΔTKΔN1L, VV CTRL and VVL-m12 against murine lung cancer LLC, CMT64, CMT167 and CMT170 cells and normal murine NIH3T3 cells. Cell death was determined by MTS assay 144 hours post-infection. EC50 values ± SEM are shown. One-way ANOVA with post hoc Tukey tests were used to assess significance (n=3/group). (C, D) Production of infectious virions after infection of murine lung cancer cell lines and normal murine cells over time. Virus production from whole cell lysates was assessed (C) and EEV production was determined via titration of viral supernatant from the same experiments (D) . Mean PFU/cell ± SEM is shown at each time-point and statistical significance determined using two-way ANOVA with Tukey’s multiple comparison post-test (n=3/group). (E) IL-12 expression after infection of murine lung cancer cell lines at an MOI of 1 PFU/cell. Supernatant was collected every 24 hours for 72 hours and assayed for IL-12 by ELISA. Data were normalized to cell number infected and displayed as ng/2×10 5 cells. n=3/group. In all cases, the mean ± SEM is shown. *p<0.05; **p<0.01; ***p<0.001; ****p<0.0001.

Article Snippet: Mouse CMT167, as well as CMT170 lung cancer cell line, human lung cancer cell lines H1299 and A549 and non-cancerous cell lines NIH3T3 (murine) and NHBE (human) were obtained from the Centre for Biomarkers & Biotherapeutics, Barts Cancer Institute, Queen Mary University of London, UK.

Techniques: Virus, Control, MTS Assay, Infection, Titration, Comparison, Expressing, Enzyme-linked Immunosorbent Assay